Intracellular measurement of Na activity using neutral carrier Na ion-selective microelectrode.
نویسندگان
چکیده
منابع مشابه
Measurement of extracellular ion fluxes using the ion-selective self-referencing microelectrode technique.
Cells from animals, plants and single cells are enclosed by a barrier called the cell membrane that separates the cytoplasm from the outside. Cell layers such as epithelia also form a barrier that separates the inside from the outside or different compartments of multicellular organisms. A key feature of these barriers is the differential distribution of ions across cell membranes or cell layer...
متن کاملIntracellular Na+ activity as a function of Na+ transport rate across a tight epithelium.
Liquid Na+ resin microelectrodes were used to measure intracellular Na+ activities (alpha iNa+) in the mammalian tight epithelium, rabbit urinary bladder. alpha iNa+ averaged 7 +/- 1 mM and was independent of Na+ transport rate over the range of 2 to 8 muA/muF. (1 mF congruent to 1 cm2 apical membrane area). After Na+ loading the cells the Na+ pump in the basolateral membrane was measurably ele...
متن کاملNa+/vacancy disordering promises high-rate Na-ion batteries
As one of the most fascinating cathode candidates for Na-ion batteries (NIBs), P2-type Na layered oxides usually exhibit various single-phase domains accompanied by different Na+/vacancy-ordered superstructures, depending on the Na concentration when explored in a limited electrochemical window. Therefore, their Na+ kinetics and cycling stability at high rates are subjected to these superstruct...
متن کاملChromium(III) ion selective electrode based on 2H-1,4-benzothioazine-2,3(4H)dione dioxime as a neutral carrier
A poly(vinyl chloride) membrane based on a new a-dioxime derivative as membrane carrier was prepared andinvestigated as a Cr(III) selective electrode. The electrode exhibits a good potentiometric response for Cr(III)over a wide concentration range 1.0 x 10-6 to 1.0 x 10-1 M with a slope 19.5+0.5 mV/decade and low detectionlimit of 8.9 x 10-7 M. It has a fast response time < 15 s. The best perfo...
متن کاملIntracellular Na+ Concentration ([Na+]i) Is Elevated in Diabetic Hearts Due to Enhanced Na+–Glucose Cotransport
BACKGROUND Intracellular Na(+) concentration ([Na(+)]i) regulates Ca(2+) cycling, contractility, metabolism, and electrical stability of the heart. [Na(+)]i is elevated in heart failure, leading to arrhythmias and oxidative stress. We hypothesized that myocyte [Na(+)]i is also increased in type 2 diabetes (T2D) due to enhanced activity of the Na(+)-glucose cotransporter. METHODS AND RESULTS T...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Japanese Journal of Physiology
سال: 1982
ISSN: 1881-1396,0021-521X
DOI: 10.2170/jjphysiol.32.997